Q99 · UPSC Prelims 2019 · Set A · Science and Technology

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What is Cas9 protein that is often mentioned in news?

A A molecular scissors used in targeted gene editing
B A biosensor used in the accurate detection of pathogens in patients
C A gene that makes plants pest-resistant
D A herbicidal substance synthesized in genetically

Correct answer: (a) A molecular scissors used in targeted gene editing

Explanation

  1. A

    A molecular scissors used in targeted gene editing

    (a) Cas9 is a molecular scissors: an RNA-guided endonuclease from CRISPR systems that cuts DNA at a targeted site so that gene editing (NHEJ or HDR) can follow. That is the official key.

  2. B

    A biosensor used in the accurate detection of pathogens in patients

    (b) A biosensor for pathogens is a different class of device (antigen tests, CRISPR diagnostics exist, but Cas9-as-such is not “a biosensor”). Not the key.

  3. C

    A gene that makes plants pest-resistant

    (c) Pest-resistance genes (Bt cry genes, for example) are not Cas9. Cas9 is the cutting enzyme, not a plant trait gene. Not the key.

  4. D

    A herbicidal substance synthesized in genetically

    (d) A herbicidal substance made in a genetically modified plant is not Cas9. Not the key.

Summary. Official key is (a). Cas9 is the CRISPR-associated nuclease that cuts targeted DNA. It is not a pathogen biosensor, not a pest-resistance gene, and not a herbicide. Honour (a).